7D5F

Left-handed G-quadruplex containing 3 bulges

Summary for 7D5F

• Entry DOI: 10.2210/pdb7d5f/pdb
• Descriptor: 3xBulge-LHG4motif (1 entity in total)
• Functional Keywords: left-handed, g-quadruplex, dna, bulge, zg4
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 8568.46

Authors

Winnerdy, F.R.,Das, P.,Ngo, K.H.,Maity, A.,Bakalar, B.,Mechulam, Y.,Schmitt, E.,Phan, A.T. (deposition date: 2020-09-25, release date: 2021-02-10, Last modification date: 2024-05-15)

Primary citation

Das, P.,Ngo, K.H.,Winnerdy, F.R.,Maity, A.,Bakalar, B.,Mechulam, Y.,Schmitt, E.,Phan, A.T.
Bulges in left-handed G-quadruplexes.
Nucleic Acids Res., 49:1724-1736, 2021

PubMed Abstract: G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

PubMed: 33503265
DOI: 10.1093/nar/gkaa1259

Experimental method

SOLUTION NMR