7D3T

Crystal structure of OSPHR2 in complex with DNA

7D3T の概要

• エントリーDOI: 10.2210/pdb7d3t/pdb
• 分子名称: Protein PHOSPHATE STARVATION RESPONSE 2, DNA (5'-D(P*GP*CP*TP*TP*GP*AP*GP*GP*AP*TP*AP*TP*CP*CP*GP*A)-3'), DNA (5'-D(P*CP*TP*CP*GP*GP*AP*TP*AP*TP*CP*CP*TP*CP*AP*AP*G)-3'), ... (4 entities in total)
• 機能のキーワード: protein phosphate starvation response 2, myb, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Oryza sativa subsp. indica (Rice); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 83483.04

構造登録者

Guan, Z.Y.,Zhang, Z.F.,Liu, Z. (登録日: 2020-09-20, 公開日: 2021-10-06, 最終更新日: 2023-11-29)

主引用文献

Guan, Z.,Zhang, Q.,Zhang, Z.,Zuo, J.,Chen, J.,Liu, R.,Savarin, J.,Broger, L.,Cheng, P.,Wang, Q.,Pei, K.,Zhang, D.,Zou, T.,Yan, J.,Yin, P.,Hothorn, M.,Liu, Z.
Mechanistic insights into the regulation of plant phosphate homeostasis by the rice SPX2 - PHR2 complex.
Nat Commun, 13:1581-1581, 2022

PubMed Abstract: Phosphate (Pi) starvation response (PHR) transcription factors play key roles in plant Pi homeostasis maintenance. They are negatively regulated by stand-alone SPX proteins, cellular receptors for inositol pyrophosphate (PP-InsP) nutrient messengers. How PP-InsP-bound SPX interacts with PHRs is poorly understood. Here, we report crystal structures of the rice SPX2/InsP/PHR2 complex and of the PHR2 DNA binding (MYB) domain in complex with target DNA at resolutions of 3.1 Å and 2.7 Å, respectively. In the SPX2/InsP/PHR2 complex, the signalling-active SPX2 assembles into a domain-swapped dimer conformation and binds two copies of PHR2, targeting both its coiled-coil (CC) oligomerisation domain and MYB domain. Our results reveal that the SPX2 senses PP-InsPs to inactivate PHR2 by establishing severe steric clashes with the PHR2 MYB domain, preventing DNA binding, and by disrupting oligomerisation of the PHR2 CC domain, attenuating promoter binding. Our findings rationalize how PP-InsPs activate SPX receptor proteins to target PHR family transcription factors.

PubMed: 35332155
DOI: 10.1038/s41467-022-29275-8

実験手法

X-RAY DIFFRACTION (2.7 Å)