7D0Y

NMR solution structures of the DNA minidumbbell formed by two CmCTG repeats at pH 5

7D0Y の概要

• エントリーDOI: 10.2210/pdb7d0y/pdb
• NMR情報: BMRB: 36379
• 分子名称: DNA (5'-D(*(DCZ)P*(5CM)P*TP*GP*CP*(5CM)P*TP*G)-3'), SODIUM ION (2 entities in total)
• 機能のキーワード: minidumbbell, dna, methylation, cctg, 5-methylcytosine, ph 5
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2441.59

構造登録者

Wan, L.,Guo, P.,Lam, S.L. (登録日: 2020-09-12, 公開日: 2021-02-03, 最終更新日: 2024-05-01)

主引用文献

Wan, L.,Yi, J.,Lam, S.L.,Lee, H.K.,Guo, P.
5-Methylcytosine Substantially Enhances the Thermal Stability of DNA Minidumbbells.
Chemistry, 27:6740-6747, 2021

PubMed Abstract: Minidumbbell (MDB) is a recently identified non-B DNA structure that has been proposed to associate with genetic instabilities. It also serves as a functional structural motif in DNA nanotechnology. DNA molecular switches constructed using MDBs show instant and complete structural conversions with easy manipulations. The availability of stable MDBs can broaden their applications. In this work, we found that substitutions of cytosine with 5-methylcytosine could lead to a significant enhancement in the thermal stabilities of MDBs. Consecutive methylations of cytosine in MDBs brought about cumulative stabilization with a drastic increase in the melting temperature by 23 °C. NMR solution structures of two MDBs containing 5-methylcytosine residues have been successfully determined and revealed that the enhanced stabilities resulted primarily from favorable hydrophobic contacts, more stable base pairs and enhanced base-base stackings involving the methyl group of 5-methylcytosine.

PubMed: 33501691
DOI: 10.1002/chem.202005410

実験手法

SOLUTION NMR