7CYA

Saimiri boliviensis boliviensis galectin-13 with lactose

7CYA の概要

• エントリーDOI: 10.2210/pdb7cya/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900004
• 分子名称: Galectin, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: saimiri boliviensis boliviensis galectin-13 with lactose, sugar binding protein
• 由来する生物種: Saimiri boliviensis boliviensis (Bolivian squirrel monkey)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16831.09

構造登録者

Su, J. (登録日: 2020-09-03, 公開日: 2021-06-16, 最終更新日: 2023-11-29)

主引用文献

Li, X.,Yao, Y.,Liu, T.,Gu, K.,Han, Q.,Zhang, W.,Ayala, G.J.,Liu, Y.,Na, H.,Yu, J.,Zhang, F.,Mayo, K.H.,Su, J.
Actin binding to galectin-13/placental protein-13 occurs independently of the galectin canonical ligand-binding site.
Glycobiology, 31:1219-1229, 2021

PubMed Abstract: The gene for galectin-13 (Gal-13, placental protein 13) is only present in primates, and its low expression level in maternal serum may promote preeclampsia. In the present study, we used pull-down experiments and biolayer interferometry to assess the interaction between Gal-13 and actin. These studies uncovered that human Gal-13 (hGal-13) and Saimiri boliviensis boliviensis (sGal-13) strongly bind to α- and β-/γ-actin, with Ca2+ and adenosine triphosphate, significantly enhancing the interactions. This in turn suggests that h/sGal-13 may inhibit myosin-induced contraction when vascular smooth muscle cells undergo polarization. Here, we solved the crystal structure of sGal-13 bound to lactose and found that it exists as a monomer in contrast to hGal-13 which is a dimer. The distribution of sGal-13 in HeLa cells is similar to that of hGal-13, indicating that monomeric Gal-13 is the primary form in cells. Even though sGal-13 binds to actin, hGal-13 ligand-binding site mutants do not influence hGal-13/actin binding, whereas the monomeric mutant C136S/C138S binds to actin more strongly than the wild-type hGal-13. Overall, our study demonstrates that monomeric Gal-13 binds to actin, an interaction that is independent of the galectin canonical ligand-binding site.

PubMed: 34080003
DOI: 10.1093/glycob/cwab047

実験手法

X-RAY DIFFRACTION (1.71 Å)