7CVO

crystal structure of Arabidopsis CO CCT domain in complex with NF-YB3/YC4 and FT CORE2 DNA

7CVO の概要

• エントリーDOI: 10.2210/pdb7cvo/pdb
• 分子名称: Chimera of Nuclear transcription factor Y subunit C-4 and Zinc finger protein CONSTANS, Nuclear transcription factor Y subunit B-3, FT CORE2 DNA forward strand, ... (5 entities in total)
• 機能のキーワード: constans, transcription factor, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 76669.16

構造登録者

Lv, X.,Du, J. (登録日: 2020-08-26, 公開日: 2021-06-16, 最終更新日: 2023-11-29)

主引用文献

Lv, X.,Zeng, X.,Hu, H.,Chen, L.,Zhang, F.,Liu, R.,Liu, Y.,Zhou, X.,Wang, C.,Wu, Z.,Kim, C.,He, Y.,Du, J.
Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex.
Plant Cell, 33:1182-1195, 2021

PubMed Abstract: Flowering plants sense various environmental and endogenous signals to trigger the floral transition and start the reproductive growth cycle. CONSTANS (CO) is a master transcription factor in the photoperiod floral pathway that integrates upstream signals and activates the florigen gene FLOWERING LOCUS T (FT). Here, we performed comprehensive structural and biochemical analyses to study the molecular mechanism underlying the regulation of FT by CO in Arabidopsis thaliana. We show that the four previously characterized cis-elements in the FT promoter proximal region, CORE1, CORE2, P1, and P2, are all direct CO binding sites. Structural analysis of CO in complex with NUCLEAR FACTOR-YB/YC (NF-YB/YC) and the CORE2 or CORE1 elements revealed the molecular basis for the specific recognition of the shared TGTG motifs. Biochemical analysis suggested that CO might form a homomultimeric assembly via its N-terminal B-Box domain and simultaneously occupy multiple cis-elements within the FT promoter. We suggest that this multivalent binding gives the CO-NF-Y complex high affinity and specificity for FT promoter binding. Overall, our data provide a detailed molecular model for the regulation of FT by the master transcription factor complex CO-NF-Y during the floral transition.

PubMed: 33693873
DOI: 10.1093/plcell/koab016

実験手法

X-RAY DIFFRACTION (2.6 Å)