7CV3
Quadruplex-duplex hybrid structure in the PIM1 gene, Form 1
Summary for 7CV3
| Entry DOI | 10.2210/pdb7cv3/pdb |
| Descriptor | PIM1 promoter, Form 1 (1 entity in total) |
| Functional Keywords | g-quadruplex, duplex, pim1, quadruplex-duplex hybrid, dna |
| Biological source | Homo sapiens |
| Total number of polymer chains | 1 |
| Total formula weight | 8506.42 |
| Authors | Winnerdy, F.R.,Tan, D.J.Y.,Lim, K.W.,Phan, A.T. (deposition date: 2020-08-25, release date: 2020-10-07, Last modification date: 2024-05-15) |
| Primary citation | Tan, D.J.Y.,Winnerdy, F.R.,Lim, K.W.,Phan, A.T. Coexistence of two quadruplex-duplex hybrids in the PIM1 gene. Nucleic Acids Res., 48:11162-11171, 2020 Cited by PubMed Abstract: The triple-negative breast cancer (TNBC), a subtype of breast cancer which lacks of targeted therapies, exhibits a poor prognosis. It was shown recently that the PIM1 oncogene is highly related to the proliferation of TNBC cells. A quadruplex-duplex hybrid (QDH) forming sequence was recently found to exist near the transcription start site of PIM1. This structure could be an attractive target for regulation of the PIM1 gene expression and thus the treatment of TNBC. Here, we present the solution structures of two QDHs that could coexist in the human PIM1 gene. Form 1 is a three-G-tetrad-layered (3+1) G-quadruplex containing a propeller loop, a lateral loop and a stem-loop made up of three G•C Watson-Crick base pairs. On the other hand, Form 2 is an anti-parallel G-quadruplex comprising two G-tetrads and a G•C•G•C tetrad; the structure has three lateral loops with the middle stem-loop made up of two Watson-Crick G•C base pairs. These structures provide valuable information for the design of G-quadruplex-specific ligands for PIM1 transcription regulation. PubMed: 32976598DOI: 10.1093/nar/gkaa752 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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