7CV3

Quadruplex-duplex hybrid structure in the PIM1 gene, Form 1

7CV3 の概要

• エントリーDOI: 10.2210/pdb7cv3/pdb
• NMR情報: BMRB: 36374
• 分子名称: PIM1 promoter, Form 1 (1 entity in total)
• 機能のキーワード: g-quadruplex, duplex, pim1, quadruplex-duplex hybrid, dna
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8506.42

構造登録者

Winnerdy, F.R.,Tan, D.J.Y.,Lim, K.W.,Phan, A.T. (登録日: 2020-08-25, 公開日: 2020-10-07, 最終更新日: 2024-05-15)

主引用文献

Tan, D.J.Y.,Winnerdy, F.R.,Lim, K.W.,Phan, A.T.
Coexistence of two quadruplex-duplex hybrids in the PIM1 gene.
Nucleic Acids Res., 48:11162-11171, 2020

PubMed Abstract: The triple-negative breast cancer (TNBC), a subtype of breast cancer which lacks of targeted therapies, exhibits a poor prognosis. It was shown recently that the PIM1 oncogene is highly related to the proliferation of TNBC cells. A quadruplex-duplex hybrid (QDH) forming sequence was recently found to exist near the transcription start site of PIM1. This structure could be an attractive target for regulation of the PIM1 gene expression and thus the treatment of TNBC. Here, we present the solution structures of two QDHs that could coexist in the human PIM1 gene. Form 1 is a three-G-tetrad-layered (3+1) G-quadruplex containing a propeller loop, a lateral loop and a stem-loop made up of three G•C Watson-Crick base pairs. On the other hand, Form 2 is an anti-parallel G-quadruplex comprising two G-tetrads and a G•C•G•C tetrad; the structure has three lateral loops with the middle stem-loop made up of two Watson-Crick G•C base pairs. These structures provide valuable information for the design of G-quadruplex-specific ligands for PIM1 transcription regulation.

PubMed: 32976598
DOI: 10.1093/nar/gkaa752

実験手法

SOLUTION NMR