7CUO

IclR transcription factor complexed with 4-hydroxybenzoic acid from Microbacterium hydrocarbonoxydans

7CUO の概要

• エントリーDOI: 10.2210/pdb7cuo/pdb
• 関連するPDBエントリー: 7DQB
• 分子名称: Transcription factor, SULFATE ION, P-HYDROXYBENZOIC ACID, ... (4 entities in total)
• 機能のキーワード: complex, gene regulation
• 由来する生物種: Microbacterium hydrocarbonoxydans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59692.20

構造登録者

Akiyama, T.,Ito, S.,Sasaki, Y.,Yajima, S. (登録日: 2020-08-23, 公開日: 2021-05-05, 最終更新日: 2023-11-29)

主引用文献

Akiyama, T.,Sasaki, Y.,Ito, S.,Yajima, S.
Structural basis of the conformational changes in Microbacterium hydrocarbonoxydans IclR transcription factor homolog due to ligand binding.
Biochim Biophys Acta Proteins Proteom, 1869:140644-140644, 2021

PubMed Abstract: Microbacterium hydrocarbonoxydans has been isolated using an unnatural acylhydrazide compound as the sole carbon source. The compound is hydrolyzed by bacterial hydrazidase, and the gene expression of the enzyme is considered to be controlled by a transcription factor of the Isocitrate lyase Regulator (IclR) family, belonging to the one-component signaling systems. Recently, we reported the crystal structure of an unliganded IclR homolog from M. hydrocarbonoxydans, named putative 4-hydroxybenzoate response regulator (pHbrR), which has a unique homotetramer conformation. In this study, we report the crystal structure of pHbrR complexed with 4-hydroxybenzoic acid, the catalytic product of hydrazidase, at 2.0 Å resolution. pHbrR forms a homodimer with multimeric rearrangement in the unliganded state. Gel filtration column chromatography results suggested dimer-tetramer rearrangement. We observed conformational change in the loop region covering the ligand-binding site, and domain rearrangements in the monomer. This study reports the first liganded IclR family protein structure that demonstrates large structural rearrangements between liganded and unliganded proteins, which may represent a general model for IclRs.

PubMed: 33716191
DOI: 10.1016/j.bbapap.2021.140644

実験手法

X-RAY DIFFRACTION (2 Å)