7CTR

Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H

7CTR の概要

• エントリーDOI: 10.2210/pdb7ctr/pdb
• 分子名称: Alpha/beta hydrolase family protein, 1,4-DIETHYLENE DIOXIDE (3 entities in total)
• 機能のキーワード: protein engineering, polyesterase, disulfide bond, metal binding, hydrolase
• 由来する生物種: Saccharomonospora viridis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58075.39

構造登録者

Emori, M.,Numoto, N.,Senga, A.,Bekker, G.J.,Kamiya, N.,Ito, N.,Kawai, F.,Oda, M. (登録日: 2020-08-20, 公開日: 2021-02-03, 最終更新日: 2023-11-29)

• 主引用文献: Emori, M.,Numoto, N.,Senga, A.,Bekker, G.J.,Kamiya, N.,Kobayashi, Y.,Ito, N.,Kawai, F.,Oda, M.; Structural basis of mutants of PET-degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability.; Proteins, 89:502-511, 2021; PubMed: 33340163; DOI: 10.1002/prot.26034

実験手法

X-RAY DIFFRACTION (1.2 Å)