7CTG

Human Origin Recognition Complex, ORC1-5 State I

7CTG の概要

• エントリーDOI: 10.2210/pdb7ctg/pdb
• 関連するPDBエントリー: 7CTE 7CTF 7CTG
• EMDBエントリー: 30462 30463 30464 30467
• 分子名称: Origin recognition complex subunit 1, Origin recognition complex subunit 2, Origin recognition complex subunit 3, ... (6 entities in total)
• 機能のキーワード: dna replication initiation, origin recognition complex, cryo-em, autoinhibition conformation, replication
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 347736.84

構造登録者

Cheng, J.,Li, N.,Wang, X.,Hu, J.,Zhai, Y.,Gao, N. (登録日: 2020-08-18, 公開日: 2021-01-06, 最終更新日: 2024-03-27)

主引用文献

Cheng, J.,Li, N.,Wang, X.,Hu, J.,Zhai, Y.,Gao, N.
Structural insight into the assembly and conformational activation of human origin recognition complex.
Cell Discov, 6:88-88, 2020

PubMed Abstract: The function of the origin recognition complex (ORC) in DNA replication is highly conserved in recognizing and marking the initiation sites. The detailed molecular mechanisms by which human ORC is reconfigured into a state competent for origin association remain largely unknown. Here, we present structural characterizations of human ORC1-5 and ORC2-5 assemblies. ORC2-5 exhibits a tightly autoinhibited conformation with the winged-helix domain of ORC2 completely blocking the central DNA-binding channel. The binding of ORC1 partially relieves the autoinhibitory effect of ORC2-5 through remodeling ORC2-WHD, which makes ORC2-WHD away from the central channel creating a still autoinhibited but more dynamic structure. In particular, the AAA+ domain of ORC1 is highly flexible to sample a variety of conformations from inactive to potentially active states. These results provide insights into the detailed mechanisms regulating the autoinhibition of human ORC and its subsequent activation for DNA binding.

PubMed: 33298899
DOI: 10.1038/s41421-020-00232-3

実験手法

ELECTRON MICROSCOPY (5 Å)