7CT8

Crystal structure of apo CmoB from Vibrio Vulnificus

7CT8 の概要

• エントリーDOI: 10.2210/pdb7ct8/pdb
• 分子名称: tRNA U34 carboxymethyltransferase (2 entities in total)
• 機能のキーワード: carboxymethyl transferase, trna modification, transferase
• 由来する生物種: Vibrio vulnificus MO6-24/O
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76787.59

構造登録者

Kim, J.,Jeong, S. (登録日: 2020-08-18, 公開日: 2021-03-03, 最終更新日: 2023-11-29)

主引用文献

Jeong, S.,Kim, J.
Structural snapshots of CmoB in various states during wobble uridine modification of tRNA.
Biochem.Biophys.Res.Commun., 534:604-609, 2021

PubMed Abstract: CmoB utilizes carboxy-S-adenosyl-l-methionine (CxSAM) to carry out unusual carboxymethyl transfer to form 5-carboxymethoxyuridine (cmoU) of several tRNA species in Gram-negative bacteria. In this report, we present three X-ray crystal structures of CmoB from Vibrio vulnificus representing different states in the course of the reaction pathway; i.e., apo-, substrate-bound, and product-bound forms. Especially, the crystal structure of apo-CmoB unveils a novel open state of the enzyme, capturing unprecedented conformational dynamics around the substrate-binding site. The apo-structure demonstrates that the open conformation favors the release of CxSAM thus representing an inactive form. Our crystal structures of CmoB complexed with CxSAM and S-adenosyl-l-homocysteine (SAH) and combined binding assay results support the proposed mechanism underlying the cofactor selectivity, where CmoB preferentially senses negative charge around amino acid residues Lys-91, Tyr-200, and Arg-315.

PubMed: 33213836
DOI: 10.1016/j.bbrc.2020.11.033

実験手法

X-RAY DIFFRACTION (2.1 Å)