7CSX

Crystal structure of the N-terminal tandem RRM domains of RBM45

7CSX の概要

• エントリーDOI: 10.2210/pdb7csx/pdb
• 分子名称: RNA-binding protein 45 (2 entities in total)
• 機能のキーワード: rna-binding, rrm, als, ftld, rna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21749.70

構造登録者

Chen, X.,Yang, Z.,Wang, W.,Wang, M. (登録日: 2020-08-17, 公開日: 2021-02-03, 最終更新日: 2023-11-29)

主引用文献

Chen, X.,Yang, Z.,Wang, W.,Qian, K.,Liu, M.,Wang, J.,Wang, M.
Structural basis for RNA recognition by the N-terminal tandem RRM domains of human RBM45.
Nucleic Acids Res., 49:2946-2958, 2021

PubMed Abstract: RBM45 is an RNA-binding protein involved in neural development, whose aggregation is associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar dementia (FTLD). However, the mechanisms of RNA-binding and aggregation of RBM45 remain unelucidated. Here, we report the crystal structure of the N-terminal tandem RRM domains of human RBM45 in complex with single-stranded DNA (ssDNA). Our structural and biochemical results revealed that both the RRM1 and RRM2 of RBM45 recognized the GAC sequence of RNA/ssDNA. Two aromatic residues and an arginine residue in each RRM were critical for RNA-binding, and the interdomain linker was also involved in RNA-binding. Two RRMs formed a pair of antiparallel RNA-binding sites, indicating that the N-terminal tandem RRM domains of RBM45 bound separate GAC motifs in one RNA strand or GAC motifs in different RNA strands. Our findings will be helpful in the identification of physiologic targets of RBM45 and provide evidence for understanding the physiologic and pathologic functions of RBM45.

PubMed: 33577684
DOI: 10.1093/nar/gkab075

実験手法

X-RAY DIFFRACTION (2.5 Å)