7CPJ

ycbZ-stalled 70S ribosome

これはPDB形式変換不可エントリーです。

7CPJ の概要

• エントリーDOI: 10.2210/pdb7cpj/pdb
• EMDBエントリー: 30431
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (58 entities in total)
• 機能のキーワード: ribosome, antibiotic
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2197799.22

構造登録者

Yokoyama, T.,Shirouzu, M.,Ito, T. (登録日: 2020-08-07, 公開日: 2021-12-08, 最終更新日: 2025-02-12)

主引用文献

Fujita, T.,Yokoyama, T.,Shirouzu, M.,Taguchi, H.,Ito, T.,Iwasaki, S.
The landscape of translational stall sites in bacteria revealed by monosome and disome profiling.
Rna, 28:290-302, 2022

PubMed Abstract: Ribosome pauses are associated with various cotranslational events and determine the fate of mRNAs and proteins. Thus, the identification of precise pause sites across the transcriptome is desirable; however, the landscape of ribosome pauses in bacteria remains ambiguous. Here, we harness monosome and disome (or collided ribosome) profiling strategies to survey ribosome pause sites in Compared to eukaryotes, ribosome collisions in bacteria showed remarkable differences: a low frequency of disomes at stop codons, collisions occurring immediately after 70S assembly on start codons, and shorter queues of ribosomes trailing upstream. The pause sites corresponded with the biochemical validation by integrated nascent chain profiling (iNP) to detect polypeptidyl-tRNA, an elongation intermediate. Moreover, the subset of those sites showed puromycin resistance, presenting slow peptidyl transfer. Among the identified sites, the ribosome pause at Asn586 of was validated by biochemical reporter assay, tRNA sequencing (tRNA-seq), and cryo-electron microscopy (cryo-EM) experiments. Our results provide a useful resource for ribosome stalling sites in bacteria.

PubMed: 34906996
DOI: 10.1261/rna.078188.120

実験手法

ELECTRON MICROSCOPY (3.3 Å)