7COJ
Crystal structure of the b-carbonic anhydrase CafA of the fungal pathogen Aspergillus fumigatus
Summary for 7COJ
| Entry DOI | 10.2210/pdb7coj/pdb |
| Descriptor | Carbonic anhydrase, ZINC ION, 5-ACETAMIDO-1,3,4-THIADIAZOLE-2-SULFONAMIDE, ... (4 entities in total) |
| Functional Keywords | b-class carbonic anhydrase, cafa, zinc metalloenzyme, aspergillus fumigatus, lyase |
| Biological source | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) |
| Total number of polymer chains | 4 |
| Total formula weight | 124620.68 |
| Authors | |
| Primary citation | Kim, S.,Yeon, J.,Sung, J.,Jin, M.S. Crystal Structure of beta-Carbonic Anhydrase CafA from the Fungal Pathogen Aspergillus fumigatus . Mol.Cells, 43:831-840, 2020 Cited by PubMed Abstract: The β-class of carbonic anhydrases (β-CAs) are zinc metalloenzymes widely distributed in the fungal kingdom that play essential roles in growth, survival, differentiation, and virulence by catalyzing the reversible interconversion of carbon dioxide (CO) and bicarbonate (HCO). Herein, we report the biochemical and crystallographic characterization of the β-CA CafA from the fungal pathogen , the main causative agent of invasive aspergillosis. CafA exhibited apparent CO hydration activity in neutral to weak alkaline conditions, but little activity at acidic pH. The high-resolution crystal structure of CafA revealed a tetramer comprising a dimer of dimers, in which the catalytic zinc ion is tetrahedrally coordinated by three conserved residues (C119, H175, C178) and an acetate anion presumably acquired from the crystallization solution, indicating a freely accessible ″open″ conformation. Furthermore, knowledge of the structure of CafA in complex with the potent inhibitor acetazolamide, together with its functional intolerance of nitrate (NO) ions, could be exploited to develop new antifungal agents for the treatment of invasive aspergillosis. PubMed: 32975213DOI: 10.14348/molcells.2020.0168 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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