7CN9

Cryo-EM structure of SARS-CoV-2 Spike ectodomain

7CN9 の概要

• エントリーDOI: 10.2210/pdb7cn9/pdb
• EMDBエントリー: 30419
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: sars-cov-2, spike, fully glycosylated, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 385298.72

構造登録者

Ho, M.,Chang, Y.,Wang, C.,Wu, Y.,Huang, H.,Chen, T.,Lo, J.M.,Chen, X.,Ma, C. (登録日: 2020-07-30, 公開日: 2020-08-26, 最終更新日: 2025-06-18)

主引用文献

Liu, Y.M.,Shahed-Al-Mahmud, M.,Chen, X.,Chen, T.H.,Liao, K.S.,Lo, J.M.,Wu, Y.M.,Ho, M.C.,Wu, C.Y.,Wong, C.H.,Jan, J.T.,Ma, C.
A Carbohydrate-Binding Protein from the Edible Lablab Beans Effectively Blocks the Infections of Influenza Viruses and SARS-CoV-2.
Cell Rep, 32:108016-108016, 2020

PubMed Abstract: The influenza virus hemagglutinin (HA) and coronavirus spike (S) protein mediate virus entry. HA and S proteins are heavily glycosylated, making them potential targets for carbohydrate binding agents such as lectins. Here, we show that the lectin FRIL, isolated from hyacinth beans (Lablab purpureus), has anti-influenza and anti-SARS-CoV-2 activity. FRIL can neutralize 11 representative human and avian influenza strains at low nanomolar concentrations, and intranasal administration of FRIL is protective against lethal H1N1 infection in mice. FRIL binds preferentially to complex-type N-glycans and neutralizes viruses that possess complex-type N-glycans on their envelopes. As a homotetramer, FRIL is capable of aggregating influenza particles through multivalent binding and trapping influenza virions in cytoplasmic late endosomes, preventing their nuclear entry. Remarkably, FRIL also effectively neutralizes SARS-CoV-2, preventing viral protein production and cytopathic effect in host cells. These findings suggest a potential application of FRIL for the prevention and/or treatment of influenza and COVID-19.

PubMed: 32755598
DOI: 10.1016/j.celrep.2020.108016

実験手法

ELECTRON MICROSCOPY (4.7 Å)