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7CHR

AcrIF9

Summary for 7CHR
Entry DOI10.2210/pdb7chr/pdb
Descriptoranti-CRISPR AcrIF9 (2 entities in total)
Functional Keywordsanti-crispr acrif9, rna binding protein
Biological sourcePhotobacterium damselae
Total number of polymer chains1
Total formula weight8885.05
Authors
Kim, G.E.,Park, H.H. (deposition date: 2020-07-06, release date: 2020-12-23, Last modification date: 2024-03-27)
Primary citationKim, G.E.,Lee, S.Y.,Park, H.H.
A high-resolution (1.2 angstrom ) crystal structure of the anti-CRISPR protein AcrIF9.
Febs Open Bio, 10:2532-2540, 2020
Cited by
PubMed Abstract: Prokaryotic adaptive immunity by CRISPR-Cas systems, which confer resistance to foreign genetic elements, has been used by bacteria to combat viruses. To cope, viruses evolved multiple anti-CRISPR proteins, which can inhibit system function through various mechanisms. Although the structures and mechanisms of several anti-CRISPR proteins have been elucidated, those of the AcrIF9 family have not yet been identified. To understand the molecular basis underlying AcrIF9 anti-CRISPR function, we determined the 1.2 Å crystal structure of AcrIF9. Structural and biochemical studies showed that AcrIF9 exists in monomeric form in solution and can directly interact with DNA using a positively charged cleft. Based on analysis of the structure, we suggest part of the anti-CRISPR molecular mechanism by AcrIF9.
PubMed: 32990416
DOI: 10.1002/2211-5463.12986
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.21 Å)
Structure validation

238895

數據於2025-07-16公開中

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