7CHR
AcrIF9
Summary for 7CHR
| Entry DOI | 10.2210/pdb7chr/pdb |
| Descriptor | anti-CRISPR AcrIF9 (2 entities in total) |
| Functional Keywords | anti-crispr acrif9, rna binding protein |
| Biological source | Photobacterium damselae |
| Total number of polymer chains | 1 |
| Total formula weight | 8885.05 |
| Authors | Kim, G.E.,Park, H.H. (deposition date: 2020-07-06, release date: 2020-12-23, Last modification date: 2024-03-27) |
| Primary citation | Kim, G.E.,Lee, S.Y.,Park, H.H. A high-resolution (1.2 angstrom ) crystal structure of the anti-CRISPR protein AcrIF9. Febs Open Bio, 10:2532-2540, 2020 Cited by PubMed Abstract: Prokaryotic adaptive immunity by CRISPR-Cas systems, which confer resistance to foreign genetic elements, has been used by bacteria to combat viruses. To cope, viruses evolved multiple anti-CRISPR proteins, which can inhibit system function through various mechanisms. Although the structures and mechanisms of several anti-CRISPR proteins have been elucidated, those of the AcrIF9 family have not yet been identified. To understand the molecular basis underlying AcrIF9 anti-CRISPR function, we determined the 1.2 Å crystal structure of AcrIF9. Structural and biochemical studies showed that AcrIF9 exists in monomeric form in solution and can directly interact with DNA using a positively charged cleft. Based on analysis of the structure, we suggest part of the anti-CRISPR molecular mechanism by AcrIF9. PubMed: 32990416DOI: 10.1002/2211-5463.12986 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.21 Å) |
Structure validation
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