7CHR

AcrIF9

7CHR の概要

• エントリーDOI: 10.2210/pdb7chr/pdb
• 分子名称: anti-CRISPR AcrIF9 (2 entities in total)
• 機能のキーワード: anti-crispr acrif9, rna binding protein
• 由来する生物種: Photobacterium damselae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8885.05

構造登録者

Kim, G.E.,Park, H.H. (登録日: 2020-07-06, 公開日: 2020-12-23, 最終更新日: 2024-03-27)

主引用文献

Kim, G.E.,Lee, S.Y.,Park, H.H.
A high-resolution (1.2 angstrom ) crystal structure of the anti-CRISPR protein AcrIF9.
Febs Open Bio, 10:2532-2540, 2020

PubMed Abstract: Prokaryotic adaptive immunity by CRISPR-Cas systems, which confer resistance to foreign genetic elements, has been used by bacteria to combat viruses. To cope, viruses evolved multiple anti-CRISPR proteins, which can inhibit system function through various mechanisms. Although the structures and mechanisms of several anti-CRISPR proteins have been elucidated, those of the AcrIF9 family have not yet been identified. To understand the molecular basis underlying AcrIF9 anti-CRISPR function, we determined the 1.2 Å crystal structure of AcrIF9. Structural and biochemical studies showed that AcrIF9 exists in monomeric form in solution and can directly interact with DNA using a positively charged cleft. Based on analysis of the structure, we suggest part of the anti-CRISPR molecular mechanism by AcrIF9.

PubMed: 32990416
DOI: 10.1002/2211-5463.12986

実験手法

X-RAY DIFFRACTION (1.21 Å)