7CHK

Cryo-EM Structure of Apple Latent Spherical Virus (ALSV)

7CHK の概要

• エントリーDOI: 10.2210/pdb7chk/pdb
• EMDBエントリー: 30375
• 分子名称: VP20 protein, VP24 protein, VP25 protein (3 entities in total)
• 機能のキーワード: cheravirus, capsid stabilization, genome release, single particle cryo-em, structural protein
• 由来する生物種: Apple latent spherical virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 65631.92

構造登録者

Naitow, H.,Hamaguchi, T.,Maki-Yonekura, S.,Isogai, M.,Yoshikawa, N.,Yonekura, K. (登録日: 2020-07-06, 公開日: 2020-11-04, 最終更新日: 2024-03-27)

主引用文献

Naitow, H.,Hamaguchi, T.,Maki-Yonekura, S.,Isogai, M.,Yoshikawa, N.,Yonekura, K.
Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release.
Commun Biol, 3:488-488, 2020

PubMed Abstract: Picorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable enough to allow its release. However, molecular mechanisms remain obscure. Here, we report a structure of a picorna-like plant virus, apple latent spherical virus, at 2.87 Å resolution by single-particle cryo-electron microscopy (cryo-EM) with a cold-field emission beam. The cryo-EM map reveals a unique structure composed of three capsid proteins Vp25, Vp20, and Vp24. Strikingly Vp25 has a long N-terminal extension, which substantially stabilises the capsid frame of Vp25 and Vp20 subunits. Cryo-EM images also resolve RNA genome leaking from a pentameric protrusion of Vp24 subunits. The structures and observations suggest that genome release occurs through occasional opening of the Vp24 subunits, possibly suppressed to a low frequency by the rigid frame of the other subunits.

PubMed: 32887929
DOI: 10.1038/s42003-020-01217-4

実験手法

ELECTRON MICROSCOPY (2.87 Å)