7CGN

The overall structure of the MlaFEDB complex in ATP-bound EQtall conformation (Mutation of E170Q on MlaF)

7CGN の概要

• エントリーDOI: 10.2210/pdb7cgn/pdb
• EMDBエントリー: 30358
• 分子名称: Lipid asymmetry maintenance ABC transporter permease subunit MlaE, Phospholipid ABC transporter ATP-binding protein MlaF, Lipid asymmetry maintenance protein MlaB, ... (5 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 253979.74

構造登録者

Chi, X.M.,Fan, Q.X.,Zhang, Y.Y.,Liang, K.,Zhou, Q.,Li, Y.Y. (登録日: 2020-07-01, 公開日: 2020-09-09, 最終更新日: 2024-03-27)

主引用文献

Chi, X.,Fan, Q.,Zhang, Y.,Liang, K.,Wan, L.,Zhou, Q.,Li, Y.
Structural mechanism of phospholipids translocation by MlaFEDB complex.
Cell Res., 30:1127-1135, 2020

PubMed Abstract: In Gram-negative bacteria, phospholipids are major components of the inner membrane and the inner leaflet of the outer membrane, playing an essential role in forming the unique dual-membrane barrier to exclude the entry of most antibiotics. Understanding the mechanisms of phospholipid translocation between the inner and outer membrane represents one of the major challenges surrounding bacterial phospholipid homeostasis. The conserved MlaFEDB complex in the inner membrane functions as an ABC transporter to drive the translocation of phospholipids between the inner membrane and the periplasmic protein MlaC. However, the mechanism of phospholipid translocation remains elusive. Here we determined three cryo-EM structures of MlaFEDB from Escherichia coli in its nucleotide-free and ATP-bound conformations, and performed extensive functional studies to verify and extend our findings from structural analyses. Our work reveals unique structural features of the entire MlaFEDB complex, six well-resolved phospholipids in three distinct cavities, and large-scale conformational changes upon ATP binding. Together, these findings define the cycle of structural rearrangement of MlaFEDB in action, and suggest that MlaFEDB uses an extrusion mechanism to extract and release phospholipids through the central translocation cavity.

PubMed: 32884137
DOI: 10.1038/s41422-020-00404-6

実験手法

ELECTRON MICROSCOPY (4.3 Å)