7CFZ

SH3 domain of NADPH oxidase activator 1

Summary for 7CFZ

• Entry DOI: 10.2210/pdb7cfz/pdb
• Descriptor: NADPH oxidase activator 1 (2 entities in total)
• Functional Keywords: sh3, noxa1, nadph oxidase activator 1, cytosolic protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 14222.04

Authors

Kim, M.,Park, J.H.,Attri, P.,Lee, W. (deposition date: 2020-06-29, release date: 2021-07-07, Last modification date: 2023-11-29)

Primary citation

Attri, P.,Park, J.H.,De Backer, J.,Kim, M.,Yun, J.H.,Heo, Y.,Dewilde, S.,Shiratani, M.,Choi, E.H.,Lee, W.,Bogaerts, A.
Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress: An experimental and computational study.
Int.J.Biol.Macromol., 163:2405-2414, 2020

PubMed Abstract: NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.

PubMed: 32961197
DOI: 10.1016/j.ijbiomac.2020.09.120

Experimental method

X-RAY DIFFRACTION (1.89 Å)