7CF8

PfkB(Mycobacterium marinum)

7CF8 の概要

• エントリーDOI: 10.2210/pdb7cf8/pdb
• 分子名称: Fructokinase, PfkB, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: pfkb, kinase, momer, sandwich, oxidoreductase, hydrolase
• 由来する生物種: Mycobacterium marinum (strain ATCC BAA-535 / M)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 190817.80

構造登録者

Li, J.,Gao, B.,Ji, R. (登録日: 2020-06-24, 公開日: 2021-06-30, 最終更新日: 2023-11-29)

主引用文献

Gao, B.,Ji, R.,Li, Z.,Su, X.,Li, H.,Sun, Y.,Ji, C.,Gan, J.,Li, J.
Structural analysis and functional study of phosphofructokinase B (PfkB) from Mycobacterium marinum.
Biochem.Biophys.Res.Commun., 579:129-135, 2021

PubMed Abstract: Phosphofructokinase B (PfkB) belongs to the ribokinase family, which uses the phosphorylated sugar as substrate, and catalyzes fructose-6-phosphate into fructose-1,6-diphosphate. However, the structural basis of Mycobacterium marinum PfkB is not clear. Here, we found that the PfkB protein was monomeric in solution, which was different from most enzymes in this family. The crystal structure of PfkB protein from M. marinum was solved at a resolution of 2.21 Å. The PfkB structure consists of two domains, a major three-layered α/β/α sandwich-like domain characteristic of the ribokinase-like superfamily, and a second domain composed of four-stranded β sheets. Structural comparison analysis suggested that residues G236, A237, G238, and D239 could be critical for ATP catalysis and substrate binding of PfkB. Our current work provides new insights into understanding the mechanism of the glycolysis in M. marinum.

PubMed: 34597996
DOI: 10.1016/j.bbrc.2021.09.051

実験手法

X-RAY DIFFRACTION (2.21 Å)