7CF6

Crystal structure of Beta-aspartyl dipeptidase from thermophilic keratin degrading Fervidobacterium islandicum AW-1 in complex with beta-Asp-Leu dipeptide

7CF6 の概要

• エントリーDOI: 10.2210/pdb7cf6/pdb
• 関連するPDBエントリー: 7CDH
• 分子名称: Isoaspartyl dipeptidase, GLYCEROL, (2S)-2-[[(3S)-3-azanyl-4-oxidanyl-4-oxidanylidene-butanoyl]amino]-4-methyl-pentanoic acid, ... (6 entities in total)
• 機能のキーワード: aspartyldipeptidase, thermophilic, feather-degrading bacterial protein, metal binding protein
• 由来する生物種: Fervidobacterium islandicum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 174089.90

構造登録者

Dhanasingh, I.,La, J.W.,Lee, D.W.,Lee, S.H. (登録日: 2020-06-24, 公開日: 2020-12-30, 最終更新日: 2023-11-29)

主引用文献

La, J.W.,Dhanasingh, I.,Jang, H.,Lee, S.H.,Lee, D.W.
Functional Characterization of Primordial Protein Repair Enzyme M38 Metallo-Peptidase From Fervidobacterium islandicum AW-1.
Front Mol Biosci, 7:600634-600634, 2020

PubMed Abstract: The NA23_RS08100 gene of AW-1 encodes a keratin-degrading β-aspartyl peptidase (BAP) that is highly expressed under starvation conditions. Herein, we expressed the gene in , purified the recombinant enzyme to homogeneity, and investigated its function. The 318 kDa recombinant BAP enzyme exhibited maximal activity at 80°C and pH 7.0 in the presence of Zn. Size-exclusion chromatography revealed that the native enzyme is an octamer comprising a tetramer of dimers; this was further supported by determination of its crystal structure at 2.6 Å resolution. Consistently, the structure of BAP revealed three additional salt bridges in each dimer, involving 12 ionic interactions that might contribute to its high thermostability. In addition, the co-crystal structure containing the substrate analog -carbobenzoxy-β-Asp-Leu at 2.7 Å resolution revealed binuclear Zn-mediated substrate binding, suggesting that BAP is a hyperthermophilic type-I IadA, in accordance with sequence-based phylogenetic analysis. Indeed, complementation of a Leu auxotrophic mutant strain (Δ and Δ) with BAP enabled the mutant strain to grow on isoAsp-Leu peptides. Remarkably, LC-MS/MS analysis of soluble keratin hydrolysates revealed that BAP not only cleaves the C-terminus of isoAsp residues but also has a relatively broad substrate specificity toward α-peptide bonds. Moreover, heat shock-induced protein aggregates retarded bacterial growth, but expression of BAP alleviated the growth defect by degrading damaged proteins. Taken together, these results suggest that the viability of hyperthermophiles under stressful conditions may rely on the activity of BAP within cellular protein repair systems.

PubMed: 33392259
DOI: 10.3389/fmolb.2020.600634

実験手法

X-RAY DIFFRACTION (2.75 Å)