7C9T

Echovirus 30 A-particle

7C9T の概要

• エントリーDOI: 10.2210/pdb7c9t/pdb
• EMDBエントリー: 30316
• 分子名称: VP1, VP2, VP3 (3 entities in total)
• 機能のキーワード: echovirus b, altered particle, virus
• 由来する生物種: Echovirus E30; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 88127.04

構造登録者

Wang, K.,Zhu, L.,Sun, Y.,Li, M.,Zhao, X.,Cui, L.,Zhang, L.,Gao, G.,Zhai, W.,Zhu, F.,Rao, Z.,Wang, X. (登録日: 2020-06-07, 公開日: 2020-07-29, 最終更新日: 2024-03-27)

主引用文献

Wang, K.,Zhu, L.,Sun, Y.,Li, M.,Zhao, X.,Cui, L.,Zhang, L.,Gao, G.F.,Zhai, W.,Zhu, F.,Rao, Z.,Wang, X.
Structures of Echovirus 30 in complex with its receptors inform a rational prediction for enterovirus receptor usage.
Nat Commun, 11:4421-4421, 2020

PubMed Abstract: Receptor usage that determines cell tropism and drives viral classification closely correlates with the virus structure. Enterovirus B (EV-B) consists of several subgroups according to receptor usage, among which echovirus 30 (E30), a leading causative agent for human aseptic meningitis, utilizes FcRn as an uncoating receptor. However, receptors for many EVs remain unknown. Here we analyzed the atomic structures of E30 mature virion, empty- and A-particles, which reveals serotype-specific epitopes and striking conformational differences between the subgroups within EV-Bs. Of these, the VP1 BC loop markedly distinguishes E30 from other EV-Bs, indicative of a role as a structural marker for EV-B. By obtaining cryo-electron microscopy structures of E30 in complex with its receptor FcRn and CD55 and comparing its homologs, we deciphered the underlying molecular basis for receptor recognition. Together with experimentally derived viral receptor identifications, we developed a structure-based in silico algorithm to inform a rational prediction for EV receptor usage.

PubMed: 32887891
DOI: 10.1038/s41467-020-18251-9

実験手法

ELECTRON MICROSCOPY (2.9 Å)