7C81

E30 F-particle in complex with 6C5

7C81 の概要

• エントリーDOI: 10.2210/pdb7c81/pdb
• EMDBエントリー: 30304
• 分子名称: VP1, VP2, VP3, ... (7 entities in total)
• 機能のキーワード: mab, 6c5, e30, virus-immune system complex, virus/immune system
• 由来する生物種: Echovirus E30; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 142896.97

構造登録者

Wang, K.,Zheng, B.,Zhang, L.,Cui, L.,Su, X.,Zhang, Q.,Guo, Y.,Zhu, L.,Zhu, F.,Rao, Z.,Wang, X. (登録日: 2020-05-28, 公開日: 2020-07-29, 最終更新日: 2020-09-16)

主引用文献

Wang, K.,Zheng, B.,Zhang, L.,Cui, L.,Su, X.,Zhang, Q.,Guo, Z.,Guo, Y.,Zhang, W.,Zhu, L.,Zhu, F.,Rao, Z.,Wang, X.
Serotype specific epitopes identified by neutralizing antibodies underpin immunogenic differences in Enterovirus B.
Nat Commun, 11:4419-4419, 2020

PubMed Abstract: Echovirus 30 (E30), a serotype of Enterovirus B (EV-B), recently emerged as a major causative agent of aseptic meningitis worldwide. E30 is particularly devastating in the neonatal population and currently no vaccine or antiviral therapy is available. Here we characterize two highly potent E30-specific monoclonal antibodies, 6C5 and 4B10, which efficiently block binding of the virus to its attachment receptor CD55 and uncoating receptor FcRn. Combinations of 6C5 and 4B10 augment the sum of their individual anti-viral activities. High-resolution structures of E30-6C5-Fab and E30-4B10-Fab define the location and nature of epitopes targeted by the antibodies. 6C5 and 4B10 engage the capsid loci at the north rim of the canyon and in-canyon, respectively. Notably, these regions exhibit antigenic variability across EV-Bs, highlighting challenges in development of broad-spectrum antibodies. Our structures of these neutralizing antibodies of E30 are instructive for development of vaccines and therapeutics against EV-B infections.

PubMed: 32887892
DOI: 10.1038/s41467-020-18250-w

実験手法

ELECTRON MICROSCOPY (3.1 Å)