7C7Y

C-terminal domain of B. cereus TubY

7C7Y の概要

• エントリーDOI: 10.2210/pdb7c7y/pdb
• 分子名称: Uncharacterized protein (2 entities in total)
• 機能のキーワード: dna binding protein, dna segregation
• 由来する生物種: Bacillus cereus (strain ATCC 10987 / NRS 248)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 37692.21

構造登録者

Hayashi, I. (登録日: 2020-05-27, 公開日: 2020-10-28, 最終更新日: 2024-10-23)

主引用文献

Hayashi, I.
The C-terminal region of the plasmid partitioning protein TubY is a tetramer that can bind membranes and DNA.
J.Biol.Chem., 295:17770-17780, 2020

PubMed Abstract: Bacterial low-copy-number plasmids require partition (par) systems to ensure their stable inheritance by daughter cells. In general, these systems consist of three components: a centromeric DNA sequence, a centromere-binding protein and a nucleotide hydrolase that polymerizes and functions as a motor. Type III systems, however, segregate plasmids using three proteins: the FtsZ/tubulin-like GTPase TubZ, the centromere-binding protein TubR and the MerR-like transcriptional regulator TubY. Although the TubZ filament is sufficient to transport the TubR-centromere complex in vitro, TubY is still necessary for the stable maintenance of the plasmid. TubY contains an N-terminal DNA-binding helix-turn-helix motif and a C-terminal coiled-coil followed by a cluster of lysine residues. This study determined the crystal structure of the C-terminal domain of TubY from the Bacillus cereus pXO1-like plasmid and showed that it forms a tetrameric parallel four-helix bundle that differs from the typical MerR family proteins with a dimeric anti-parallel coiled-coil. Biochemical analyses revealed that the C-terminal tail with the conserved lysine cluster helps TubY to stably associate with the TubR-centromere complex as well as to nonspecifically bind DNA. Furthermore, this C-terminal tail forms an amphipathic helix in the presence of lipids but must oligomerize to localize the protein to the membrane in vivo. Taken together, these data suggest that TubY is a component of the nucleoprotein complex within the partitioning machinery, and that lipid membranes act as mediators of type III systems.

PubMed: 33454013
DOI: 10.1074/jbc.RA120.014705

実験手法

X-RAY DIFFRACTION (2.6 Å)