7C1Y

Pseudouridine and ADP bound structure of Pseudouridine kinase (PUKI) from Arabidopsis thaliana

7C1Y の概要

• エントリーDOI: 10.2210/pdb7c1y/pdb
• 分子名称: PfkB-like carbohydrate kinase family protein, SODIUM ION, 5-[(2~{S},3~{R},4~{S},5~{R})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]-1~{H}-pyrimidine-2,4-dione, ... (5 entities in total)
• 機能のキーワード: pseudouridine, kinase, psedouridine kinase, puki, rna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82110.57

構造登録者

Kim, S.H.,Rhee, S. (登録日: 2020-05-06, 公開日: 2020-11-18, 最終更新日: 2024-11-13)

主引用文献

Kim, S.H.,Witte, C.P.,Rhee, S.
Structural basis for the substrate specificity and catalytic features of pseudouridine kinase from Arabidopsis thaliana.
Nucleic Acids Res., 49:491-503, 2021

PubMed Abstract: RNA modifications can regulate the stability of RNAs, mRNA-protein interactions, and translation efficiency. Pseudouridine is a prevalent RNA modification, and its metabolic fate after RNA turnover was recently characterized in eukaryotes, in the plant Arabidopsis thaliana. Here, we present structural and biochemical analyses of PSEUDOURIDINE KINASE from Arabidopsis (AtPUKI), the enzyme catalyzing the first step in pseudouridine degradation. AtPUKI, a member of the PfkB family of carbohydrate kinases, is a homodimeric α/β protein with a protruding small β-strand domain, which serves simultaneously as dimerization interface and dynamic substrate specificity determinant. AtPUKI has a unique nucleoside binding site specifying the binding of pseudourine, in particular at the nucleobase, by multiple hydrophilic interactions, of which one is mediated by a loop from the small β-strand domain of the adjacent monomer. Conformational transition of the dimerized small β-strand domains containing active site residues is required for substrate specificity. These dynamic features explain the higher catalytic efficiency for pseudouridine over uridine. Both substrates bind well (similar Km), but only pseudouridine is turned over efficiently. Our studies provide an example for structural and functional divergence in the PfkB family and highlight how AtPUKI avoids futile uridine phosphorylation which in vivo would disturb pyrimidine homeostasis.

PubMed: 33290549
DOI: 10.1093/nar/gkaa1144

実験手法

X-RAY DIFFRACTION (2.0834261145 Å)