7C1M
Complex structure of tyrosinated alpha-tubulin carboxy-terminal peptide and A1aY1 binder
Summary for 7C1M
| Entry DOI | 10.2210/pdb7c1m/pdb |
| Descriptor | Nanobody binder from SSO7d library, Carboxy-terminal peptide from tyrosinated alpha-tubulin (2 entities in total) |
| Functional Keywords | tyrosinated, microtubule, binder, sso7d, yeast display, protein binding |
| Biological source | Saccharolobus solfataricus 98/2 More |
| Total number of polymer chains | 2 |
| Total formula weight | 8842.13 |
| Authors | Kesarwani, S.,Reddy, P.P.,Sirajuddin, M.,Das, R. (deposition date: 2020-05-05, release date: 2020-09-02, Last modification date: 2024-05-15) |
| Primary citation | Kesarwani, S.,Lama, P.,Chandra, A.,Reddy, P.P.,Jijumon, A.S.,Bodakuntla, S.,Rao, B.M.,Janke, C.,Das, R.,Sirajuddin, M. Genetically encoded live-cell sensor for tyrosinated microtubules. J.Cell Biol., 219:-, 2020 Cited by PubMed Abstract: Microtubule cytoskeleton exists in various biochemical forms in different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known to affect microtubule stability, dynamics, and interaction with MAPs and motors in a specific manner, widely known as tubulin code hypothesis. At present, there exists no tool that can specifically mark tubulin PTMs in living cells, thus severely limiting our understanding of their dynamics and cellular functions. Using a yeast display library, we identified a binder against terminal tyrosine of α-tubulin, a unique PTM site. Extensive characterization validates the robustness and nonperturbing nature of our binder as tyrosination sensor, a live-cell tubulin nanobody specific towards tyrosinated microtubules. Using this sensor, we followed nocodazole-, colchicine-, and vincristine-induced depolymerization events of tyrosinated microtubules in real time and found each distinctly perturbs the microtubule polymer. Together, our work describes a novel tyrosination sensor and its potential applications to study the dynamics of microtubule and their PTM processes in living cells. PubMed: 32886100DOI: 10.1083/jcb.201912107 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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