7C0P

Structure of proteinase K obtained in SSRF using serial crystallography

7C0P の概要

• エントリーDOI: 10.2210/pdb7c0p/pdb
• 分子名称: Proteinase K, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Parengyodontium album (Tritirachium album,Engyodontium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28998.87

構造登録者

Zhao, F.Z. (登録日: 2020-05-01, 公開日: 2020-12-09, 最終更新日: 2024-10-09)

主引用文献

Zhao, F.Z.,Sun, B.,Yu, L.,Xiao, Q.J.,Wang, Z.J.,Chen, L.L.,Liang, H.,Wang, Q.S.,He, J.H.,Yin, D.C.
A novel sample delivery system based on circular motion for in situ serial synchrotron crystallography.
Lab Chip, 20:3888-3898, 2020

PubMed Abstract: A sample delivery system is one of the key parts of serial crystallography. It is the main limiting factor affecting the application of serial crystallography. At present, although a variety of useful sample delivery systems have been developed for serial crystallography, it still remains the focus of the field to further improve the performance and efficiency of sample delivery. In existing sample delivery technologies, samples are usually delivered in linear motion. Here we show that the samples can also be delivered using circular motion, which is a novel motion mode never tested before. In this paper, we report a microfluidic rotating-target sample delivery device, which is characterized by the circular motion of the samples, and verify the performance of the device at a synchrotron radiation facility. The microfluidic rotating-target sample delivery device consists of two parts: a microfluidic sample plate and a motion control system. Sample delivery is realized by rotating the microfluidic sample plate containing in situ grown crystals. This device offers significant advantages, including a very wide adjustable range of delivery speed, low background noise, and low sample consumption. Using the microfluidic rotating-target device, we carried out in situ serial crystallography experiments with lysozyme and proteinase K as model samples at the Shanghai Synchrotron Radiation Facility, and performed structural determination based on the serial crystallographic data. The results showed that the designed device is fully compatible with the synchrotron radiation facility, and the structure determination of proteins is successful using the serial crystallographic data obtained with the device.

PubMed: 32966481
DOI: 10.1039/d0lc00443j

実験手法

X-RAY DIFFRACTION (2.15 Å)