7BZH

Solution structure of a DNA binding protein from Sulfolobus islandicus

7BZH の概要

• エントリーDOI: 10.2210/pdb7bzh/pdb
• 分子名称: Sul7s (1 entity in total)
• 機能のキーワード: archaea, crenarchaea, sulfolobus, dna binding protein
• 由来する生物種: Sulfolobus islandicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7226.36

構造登録者

Zhang, Z.,Liu, X. (登録日: 2020-04-28, 公開日: 2021-04-28, 最終更新日: 2024-05-15)

主引用文献

Huang, C.,Liu, X.,Chen, Y.,Zhou, J.,Li, W.,Ding, N.,Huang, L.,Chen, J.,Zhang, Z.
A Novel Family of Winged-Helix Single-Stranded DNA-Binding Proteins from Archaea.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: The winged helix superfamily comprises a large number of structurally related nucleic acid-binding proteins. While these proteins are often shown to bind dsDNA, few are known to bind ssDNA. Here, we report the identification and characterization of Sul7s, a novel winged-helix single-stranded DNA binding protein family highly conserved in . Sul7s from binds ssDNA with an affinity approximately 15-fold higher than that for dsDNA in vitro. It prefers binding oligo(dT) over oligo(dC) or a dG-rich 30-nt oligonucleotide, and barely binds oligo(dA). Further, binding by Sul7s inhibits DNA strand annealing, but shows little effect on the melting temperature of DNA duplexes. The solution structure of Sul7s determined by NMR shows a winged helix-turn-helix fold, consisting of three α-helices, three β-strands, and two short wings. It interacts with ssDNA via a large positively charged binding surface, presumably resulting in ssDNA deformation. Our results shed significant light on not only non-OB fold single-stranded DNA binding proteins in Archaea, but also the divergence of the winged-helix proteins in both function and structure during evolution.

PubMed: 35408816
DOI: 10.3390/ijms23073455

実験手法

SOLUTION NMR