7BWI

Solution structure of recombinant APETx1

7BWI の概要

• エントリーDOI: 10.2210/pdb7bwi/pdb
• NMR情報: BMRB: 36345
• 分子名称: Kappa-actitoxin-Ael2a (1 entity in total)
• 機能のキーワード: toxin
• 由来する生物種: Anthopleura elegantissima (Green aggregating anemone)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4561.23

構造登録者

Matsumura, K.,Kobayashi, N.,Kurita, J.,Nishimura, Y.,Yokogawa, M.,Imai, S.,Shimada, I.,Osawa, M. (登録日: 2020-04-14, 公開日: 2020-12-23, 最終更新日: 2024-10-16)

主引用文献

Matsumura, K.,Shimomura, T.,Kubo, Y.,Oka, T.,Kobayashi, N.,Imai, S.,Yanase, N.,Akimoto, M.,Fukuda, M.,Yokogawa, M.,Ikeda, K.,Kurita, J.I.,Nishimura, Y.,Shimada, I.,Osawa, M.
Mechanism of hERG inhibition by gating-modifier toxin, APETx1, deduced by functional characterization.
Bmc Mol Cell Biol, 22:3-3, 2021

PubMed Abstract: Human ether-à-go-go-related gene potassium channel 1 (hERG) is a voltage-gated potassium channel, the voltage-sensing domain (VSD) of which is targeted by a gating-modifier toxin, APETx1. APETx1 is a 42-residue peptide toxin of sea anemone Anthopleura elegantissima and inhibits hERG by stabilizing the resting state. A previous study that conducted cysteine-scanning analysis of hERG identified two residues in the S3-S4 region of the VSD that play important roles in hERG inhibition by APETx1. However, mutational analysis of APETx1 could not be conducted as only natural resources have been available until now. Therefore, it remains unclear where and how APETx1 interacts with the VSD in the resting state.

PubMed: 33413079
DOI: 10.1186/s12860-020-00337-3

実験手法

SOLUTION NMR