7BWE

Consensus Chitin binding domain

7BWE の概要

• エントリーDOI: 10.2210/pdb7bwe/pdb
• 分子名称: Chitin binding beak protein 3 (1 entity in total)
• 機能のキーワード: chitin binding protein, chitopentaose, std-nmr., sugar binding protein
• 由来する生物種: Dosidicus gigas (Humboldt squid)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7058.85

構造登録者

Mohanram, H.,Miserez, A. (登録日: 2020-04-14, 公開日: 2021-04-14, 最終更新日: 2024-05-15)

主引用文献

Heymann, D.,Mohanram, H.,Kumar, A.,Verma, C.S.,Lescar, J.,Miserez, A.
Structure of a consensus chitin-binding domain revealed by solution NMR.
J.Struct.Biol., 213:107725-107725, 2021

PubMed Abstract: Chitin-binding proteins (CBPs) are a versatile group of proteins found in almost every organism on earth. CBPs are involved in enzymatic carbohydrate degradation and also serve as templating scaffolds in the exoskeleton of crustaceans and insects. One specific chitin-binding motif found across a wide range of arthropods' exoskeletons is the "extended Rebers and Riddiford" consensus (R&R), whose mechanism of chitin binding remains unclear. Here, we report the 3D structure and molecular level interactions of a chitin-binding domain (CBD-γ) located in a CBP from the beak of the jumbo squid Dosidicus gigas. This CBP is one of four chitin-binding proteins identified in the beak mouthpart of D. gigas and is believed to interact with chitin to form a scaffold network that is infiltrated with a second set of structural proteins during beak maturation. We used solution state NMR spectroscopy to elucidate the molecular interactions between CBD-γ and the soluble chitin derivative pentaacetyl-chitopentaose (PCP), and find that folding of CBD-γ is triggered upon its interaction with PCP. To our knowledge, this is the first experimental 3D structure of a CBP containing the R&R consensus motif, which can be used as a template to understand in more details the role of the R&R motif found in a wide range of CBP-chitin complexes. The present structure also provides molecular information for biomimetic synthesis of graded biomaterials using aqueous-based chemistry and biopolymers.

PubMed: 33744410
DOI: 10.1016/j.jsb.2021.107725

実験手法

SOLUTION NMR