7BW4

Structure of the RNA-dependent RNA polymerase from SARS-CoV-2

7BW4 の概要

• エントリーDOI: 10.2210/pdb7bw4/pdb
• EMDBエントリー: 30226
• 分子名称: RNA-directed RNA polymerase, Non-structural protein 8, Non-structural protein 7, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, polymerase, cryo-em, replication
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 159032.72

構造登録者

Peng, Q.,Peng, R.,Shi, Y. (登録日: 2020-04-13, 公開日: 2020-05-27, 最終更新日: 2025-07-02)

主引用文献

Peng, Q.,Peng, R.,Yuan, B.,Zhao, J.,Wang, M.,Wang, X.,Wang, Q.,Sun, Y.,Fan, Z.,Qi, J.,Gao, G.F.,Shi, Y.
Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2.
Cell Rep, 31:107774-107774, 2020

PubMed Abstract: The ongoing global pandemic of coronavirus disease 2019 (COVID-19) has caused a huge number of human deaths. Currently, there are no specific drugs or vaccines available for this virus (SARS-CoV-2). The viral polymerase is a promising antiviral target. Here, we describe the near-atomic-resolution structure of the SARS-CoV-2 polymerase complex consisting of the nsp12 catalytic subunit and nsp7-nsp8 cofactors. This structure highly resembles the counterpart of SARS-CoV with conserved motifs for all viral RNA-dependent RNA polymerases and suggests a mechanism of activation by cofactors. Biochemical studies reveal reduced activity of the core polymerase complex and lower thermostability of individual subunits of SARS-CoV-2 compared with SARS-CoV. These findings provide important insights into RNA synthesis by coronavirus polymerase and indicate adaptation of SARS-CoV-2 toward humans with a relatively lower body temperature than the natural bat hosts.

PubMed: 32531208
DOI: 10.1016/j.celrep.2020.107774

実験手法

ELECTRON MICROSCOPY (3.7 Å)