7BVM

Crystal structure of lysozyme delivered in wheat starch

Summary for 7BVM

• Entry DOI: 10.2210/pdb7bvm/pdb
• Descriptor: Lysozyme C, CHLORIDE ION (3 entities in total)
• Functional Keywords: lysozyme, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 16328.57

Authors

Nam, K.H. (deposition date: 2020-04-11, release date: 2020-11-18, Last modification date: 2024-10-16)

Primary citation

Nam, K.H.
Polysaccharide-Based Injection Matrix for Serial Crystallography.
Int J Mol Sci, 21:-, 2020

PubMed Abstract: Serial crystallography (SX) provides an opportunity to observe the molecular dynamics of macromolecular structures at room temperature via pump-probe studies. The delivery of crystals embedded in a viscous medium via an injector or syringe is widely performed in synchrotrons or X-ray free-electron laser facilities with low repetition rates. Various viscous media have been developed; however, there are cases in which the delivery material undesirably interacts chemically or biologically with specific protein samples, or changes the stability of the injection stream, depending on the crystallization solution. Therefore, continued discovery and characterization of new delivery media is necessary for expanding future SX applications. Here, the preparation and characterization of new polysaccharide (wheat starch (WS) and alginate)-based sample delivery media are introduced for SX. Crystals embedded in a WS or alginate injection medium showed a stable injection stream at a flow rate of < 200 nL/min and low-level X-ray background scattering similar to other hydrogels. Using these media, serial millisecond crystallography (SMX) was performed, and the room temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9-2.0 Å resolutions. WS and alginate will allow an expanded application of sample delivery media in SX experiments.

PubMed: 32397185
DOI: 10.3390/ijms21093332

Experimental method

X-RAY DIFFRACTION (2 Å)