7BVI

Crystal structure of Pennisetum glaucum monodehydroascorbate reductase

7BVI の概要

• エントリーDOI: 10.2210/pdb7bvi/pdb
• 分子名称: Pennisetum glaucum monodehydroascorbate reductase, FLAVIN-ADENINE DINUCLEOTIDE, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: nucleotide binding, oxidoreductase activity, monodehydroascorbate reductase (nadh) activity, flavin adenine dinucleotide binding, oxidoreductase
• 由来する生物種: Cenchrus americanus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 190750.91

構造登録者

Sonkar, K.S.,Arulandu, A.,Achary, M.M.,Reddy, M.K. (登録日: 2020-04-10, 公開日: 2021-04-14, 最終更新日: 2023-11-29)

主引用文献

Sonkar, K.S.,Achary, V.M.M.,Sahoo, S.,Reddy, M.K.,Arockiasamy, A.
Biochemical and structural characterization of a robust and thermostable ascorbate recycling monodehydroascorbate reductase (MDHAR) from stress adapted pearl millet.
Biochem.Biophys.Res.Commun., 662:135-141, 2023

PubMed Abstract: Ascorbate (AsA) is a crucial antioxidant in plants, and its recycling is necessary for protecting cells from oxidative damage and imparting stress tolerance. The monodehydroascorbate reductase (MDHAR) enzyme of the ascorbate-glutathione pathway plays a vital role in recycling AsA from monodehydroascorbate (MDHA) radical. Pennisetum glaucum (Pg), also known as pearl millet, is known to be more tolerant to abiotic stress than other food crops, such as rice. However, the contribution of MDHAR from this sessile plant to its unique stress tolerance mechanism is not well understood. In this study, we isolated a gene encoding the MDHAR enzyme from heat stress-adapted pearl millet and characterized it using enzyme kinetics, thermal stability assays, and crystal structure determination. Our results indicate that PgMDHAR is a more robust enzyme than its rice counterpart (Oryza sativa; Os). We solved the crystal structure of PgMDHAR at 1.8 Å and found that the enzyme has a more compact structure and greater stability than OsMDHAR. Using hybrid quantum mechanics and molecular mechanics calculations, we demonstrate that the structure of PgMDHAR contributes to increased stability towards bound FAD. Overall, the higher structural stability and affinity for NADH demonstrated by PgMDHAR are expected to impart improved stress tolerance. Our findings suggest that transgenic food crops expressing MDHAR from stress-adapted pearl millet may exhibit better tolerance to oxidative stress in the unpredictable climatic conditions prevalent today.

PubMed: 37119729
DOI: 10.1016/j.bbrc.2023.04.034

実験手法

X-RAY DIFFRACTION (2.391 Å)