7BV7

INTS3 complexed with INTS6

7BV7 の概要

• エントリーDOI: 10.2210/pdb7bv7/pdb
• 分子名称: Integrator complex subunit 3, Integrator complex subunit 6 (3 entities in total)
• 機能のキーワード: dna double strand break repair ints3 ints6 ssb1, recombination
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 111243.57

構造登録者

Jia, Y.,Bharath, S.R.,Song, H. (登録日: 2020-04-09, 公開日: 2021-07-14, 最終更新日: 2024-05-29)

主引用文献

Jia, Y.,Cheng, Z.,Bharath, S.R.,Sun, Q.,Su, N.,Huang, J.,Song, H.
Crystal structure of the INTS3/INTS6 complex reveals the functional importance of INTS3 dimerization in DSB repair.
Cell Discov, 7:66-66, 2021

PubMed Abstract: SOSS1 is a single-stranded DNA (ssDNA)-binding protein complex that plays a critical role in double-strand DNA break (DSB) repair. SOSS1 consists of three subunits: INTS3, SOSSC, and hSSB1, with INTS3 serving as a scaffold to stabilize this complex. Moreover, the integrator complex subunit 6 (INTS6) participates in the DNA damage response through direct binding to INTS3, but how INTS3 interacts with INTS6, thereby impacting DSB repair, is not clear. Here, we determined the crystal structure of the C-terminus of INTS3 (INTS3c) in complex with the C-terminus of INTS6 (INTS6c) at a resolution of 2.4 Å. Structural analysis revealed that two INTS3c subunits dimerize and interact with INTS6c via conserved residues. Subsequent biochemical analyses confirmed that INTS3c forms a stable dimer and INTS3 dimerization is important for recognizing the longer ssDNA. Perturbation of INTS3c dimerization and disruption of the INTS3c/INTS6c interaction impair the DSB repair process. Altogether, these results unravel the underappreciated role of INTS3 dimerization and the molecular basis of INTS3/INTS6 interaction in DSB repair.

PubMed: 34400606
DOI: 10.1038/s41421-021-00283-0

実験手法

X-RAY DIFFRACTION (2.4 Å)