7BTK
E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA01
Summary for 7BTK
| Entry DOI | 10.2210/pdb7btk/pdb |
| Descriptor | Beta-galactosidase, 4-[[2-[(E)-2-[4-[(2S,3R,4S,5R,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxyphenyl]ethenyl]-3,3-dimethyl-2H-indol-1-yl]methyl]benzoic acid, MAGNESIUM ION, ... (7 entities in total) |
| Functional Keywords | beta_galactosidase, fluorescent probe, hydrolase |
| Biological source | Escherichia coli K-12 |
| Total number of polymer chains | 4 |
| Total formula weight | 472388.86 |
| Authors | |
| Primary citation | Gao, Y.,Hu, Y.,Liu, Q.,Li, X.,Li, X.,Kim, C.Y.,James, T.D.,Li, J.,Chen, X.,Guo, Y. Two-Dimensional Design Strategy to Construct Smart Fluorescent Probes for the Precise Tracking of Senescence. Angew.Chem.Int.Ed.Engl., 60:10756-10765, 2021 Cited by PubMed Abstract: The tracking of cellular senescence usually depends on the detection of senescence-associated β-galactosidase (SA-β-gal). Previous probes for SA-β-gal with this purpose only cover a single dimension: the accumulation of this enzyme in lysosomes. However, this is insufficient to determine the destiny of senescence because endogenous β-gal enriched in lysosomes is not only related to senescence, but also to some other physiological processes. To address this issue, we introduce our fluorescent probes including a second dimension: lysosomal pH, since de-acidification is a unique feature of the lysosomes in senescent cells. With this novel design, our probes achieved excellent discrimination of SA-β-gal from cancer-associated β-gal, which enables them to track cellular senescence as well as tissue aging more precisely. Our crystal structures of a model enzyme E. coli β-gal mutant (E537Q) complexed with each probe further revealed the structural basis for probe recognition. PubMed: 33624914DOI: 10.1002/anie.202101278 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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