7BSO

Crystal structure of the human NLRP9 pyrin domain

Summary for 7BSO

• Entry DOI: 10.2210/pdb7bso/pdb
• Descriptor: NACHT, LRR and PYD domains-containing protein 9 (2 entities in total)
• Functional Keywords: sensor component, nlrp9 inflammasome, apoptosis
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 12807.89

Authors

Park, H.H.,Ha, H.J. (deposition date: 2020-03-31, release date: 2021-02-10, Last modification date: 2023-11-29)

Primary citation

Ha, H.J.,Park, H.H.
Crystal structure of the human NLRP9 pyrin domain reveals a bent N-terminal loop that may regulate inflammasome assembly.
Febs Lett., 594:2396-2405, 2020

PubMed Abstract: Members of the NLR family pyrin domain containing (NLRPs) are pattern recognition receptors that participate in innate immunity. They form inflammasomes, which are platforms for caspase-1 recruitment and activation. The NLRP pyrin domain (PYD) is critical for the assembly of inflammasomes due to its ability to mediate protein interactions. Despite intensive structural studies on inflammasomes with PYDs, the structure of the PYD of NLRP9-the least studied member of the family-remains unknown. Herein, we report the crystal structure of the human NLRP9 PYD at 2.1 Å resolution, which reveals a kinked N-terminal loop oriented toward the interior of the helical bundle. Based on our findings, we propose a regulatory role for the kinked N-terminal loop of NLRP9 PYD in inflammasome assembly.

PubMed: 32542766
DOI: 10.1002/1873-3468.13866

Experimental method

X-RAY DIFFRACTION (2.08 Å)