7BOV

The Structure of Bacillus subtilis glycosyltransferase,Bs-YjiC

7BOV の概要

• エントリーDOI: 10.2210/pdb7bov/pdb
• 分子名称: Uncharacterized UDP-glucosyltransferase YjiC, SODIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: glycosyltransferase, transferase
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44240.60

構造登録者

Zhao, C.,Liu, B.,Zhao, N.L.,Luo, Y.Z.,Bao, R. (登録日: 2020-03-20, 公開日: 2020-05-13, 最終更新日: 2023-11-29)

主引用文献

Liu, B.,Zhao, C.,Xiang, Q.,Zhao, N.,Luo, Y.,Bao, R.
Structural and biochemical studies of the glycosyltransferase Bs-YjiC from Bacillus subtilis.
Int.J.Biol.Macromol., 166:806-817, 2021

PubMed Abstract: Glycosylation possess prominent biological and pharmacological significance in natural product and drug candidate synthesis. The glycosyltransferase YjiC, discovered from Bacillus subtilis (Bs-YjiC), shows potential applications in drug development due to its wide substrate spectrums. In order to elucidate its catalytic mechanism, we solved the crystal structure of Bs-YjiC, demonstrating that Bs-YjiC adopts a typical GT-B fold consisting of a flexible N-domain and a relatively rigid C-domain. Structural analysis coupled with site-directed mutagenesis studies revealed that site Ser277 was critical for Nucleoside Diphosphate (NDP) recognition, while Glu317, Gln318, Ser128 and Ser129 were crucial for glycosyl moiety recognition. Our results illustrate the structural basis for acceptor promiscuity in Bs-YjiC and provide a starting point for further protein engineering of Bs-YjiC in industrial and pharmaceutical applications.

PubMed: 33152360
DOI: 10.1016/j.ijbiomac.2020.10.238

実験手法

X-RAY DIFFRACTION (2.293 Å)