7BNT

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with a predicted ancestral HMA domain of Pik-1 from Oryza spp.

7BNT の概要

• エントリーDOI: 10.2210/pdb7bnt/pdb
• 分子名称: Predicted ancestral HMA domain of Pik-1 from Oryza spp., AVR-Pik protein, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: rice blast disease, plant disease resistance, effector protein, integrated hma domain, protein binding
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 26572.99

構造登録者

Bialas, A.,Langner, T.,Harant, A.,Contreras, M.P.,Stevenson, C.E.M.,Lawson, D.M.,Sklenar, J.,Kellner, R.,Moscou, M.J.,Terauchi, R.,Banfield, M.J.,Kamoun, S. (登録日: 2021-01-22, 公開日: 2021-02-17, 最終更新日: 2024-10-09)

主引用文献

Bialas, A.,Langner, T.,Harant, A.,Contreras, M.P.,Stevenson, C.E.,Lawson, D.M.,Sklenar, J.,Kellner, R.,Moscou, M.J.,Terauchi, R.,Banfield, M.J.,Kamoun, S.
Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain.
Elife, 10:-, 2021

PubMed Abstract: A subset of plant NLR immune receptors carry unconventional integrated domains in addition to their canonical domain architecture. One example is rice Pik-1 that comprises an integrated heavy metal-associated (HMA) domain. Here, we reconstructed the evolutionary history of Pik-1 and its NLR partner, Pik-2, and tested hypotheses about adaptive evolution of the HMA domain. Phylogenetic analyses revealed that the HMA domain integrated into Pik-1 before Oryzinae speciation over 15 million years ago and has been under diversifying selection. Ancestral sequence reconstruction coupled with functional studies showed that two Pik-1 allelic variants independently evolved from a weakly binding ancestral state to high-affinity binding of the blast fungus effector AVR-PikD. We conclude that for most of its evolutionary history the Pik-1 HMA domain did not sense AVR-PikD, and that different Pik-1 receptors have recently evolved through distinct biochemical paths to produce similar phenotypic outcomes. These findings highlight the dynamic nature of the evolutionary mechanisms underpinning NLR adaptation to plant pathogens.

PubMed: 34288868
DOI: 10.7554/eLife.66961

実験手法

X-RAY DIFFRACTION (1.32 Å)