7BME

Crystal structure of a R18W mutant of the DNA-binding protein RemA from Geobacillus thermodenitrificans

7BME の概要

• エントリーDOI: 10.2210/pdb7bme/pdb
• 関連するPDBエントリー: 7BM2
• 分子名称: Putative regulatory protein GTNG_1019 (2 entities in total)
• 機能のキーワード: biofilm, dna-binding, histone-like, geobacillus, dna binding protein
• 由来する生物種: Geobacillus thermodenitrificans (strain NG80-2)
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 73527.37

構造登録者

Altegoer, F.,Mrusek, D.,Bange, G. (登録日: 2021-01-20, 公開日: 2021-08-25, 最終更新日: 2024-01-31)

主引用文献

Hoffmann, T.,Mrusek, D.,Bedrunka, P.,Burchert, F.,Mais, C.N.,Kearns, D.B.,Altegoer, F.,Bremer, E.,Bange, G.
Structural and functional characterization of the bacterial biofilm activator RemA.
Nat Commun, 12:5707-5707, 2021

PubMed Abstract: Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain.

PubMed: 34588455
DOI: 10.1038/s41467-021-26005-4

実験手法

X-RAY DIFFRACTION (2.6 Å)