7BL5

pre-50S-ObgE particle

7BL5 の概要

• エントリーDOI: 10.2210/pdb7bl5/pdb
• EMDBエントリー: 12218
• 分子名称: 50S ribosomal protein L2, 50S ribosomal protein L21, 50S ribosomal protein L22, ... (39 entities in total)
• 機能のキーワード: ribosome, pre-50s, ribosome biogenesis, ribosome assembly
• 由来する生物種: Escherichia coli str. K-12 substr. MG1655; 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1490379.57

構造登録者

Hilal, T.,Nikolay, R.,Spahn, C.M.T.,Schmidt, S. (登録日: 2021-01-18, 公開日: 2021-05-12, 最終更新日: 2025-12-17)

主引用文献

Nikolay, R.,Hilal, T.,Schmidt, S.,Qin, B.,Schwefel, D.,Vieira-Vieira, C.H.,Mielke, T.,Burger, J.,Loerke, J.,Amikura, K.,Flugel, T.,Ueda, T.,Selbach, M.,Deuerling, E.,Spahn, C.M.T.
Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization.
Mol.Cell, 81:1200-1215.e9, 2021

PubMed Abstract: Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.

PubMed: 33639093
DOI: 10.1016/j.molcel.2021.02.006

実験手法

ELECTRON MICROSCOPY (3.3 Å)