7BIN

Salmonella export gate and rod refined in focussed C1 map

7BIN の概要

• エントリーDOI: 10.2210/pdb7bin/pdb
• EMDBエントリー: 12183 12190 12192
• 分子名称: Flagellar biosynthetic protein FliP, Flagellar biosynthetic protein FliR, Flagellar biosynthetic protein FliQ, ... (8 entities in total)
• 機能のキーワード: bacterial flagellum rod bacterial flagellum export gate basal body, protein transport
• 由来する生物種: Salmonella typhi; 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 1225015.42

構造登録者

Johnson, S.,Furlong, E.,Lea, S.M. (登録日: 2021-01-12, 公開日: 2021-05-05, 最終更新日: 2024-07-10)

主引用文献

Johnson, S.,Furlong, E.J.,Deme, J.C.,Nord, A.L.,Caesar, J.J.E.,Chevance, F.F.V.,Berry, R.M.,Hughes, K.T.,Lea, S.M.
Molecular structure of the intact bacterial flagellar basal body.
Nat Microbiol, 6:712-721, 2021

PubMed Abstract: The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.

PubMed: 33931760
DOI: 10.1038/s41564-021-00895-y

実験手法

ELECTRON MICROSCOPY (3.2 Å)