7BIM
A de novo designed nonameric coiled coil, CC-Type2-(GgLaId)4
Summary for 7BIM
| Entry DOI | 10.2210/pdb7bim/pdb |
| Related | 7A1T 7BAS 7BAT 7BAU 7BAV 7BAW |
| Descriptor | Nonameric de novo coiled coil CC-Type2-(GgLaId)4, GLYCEROL, ISOPROPYL ALCOHOL, ... (4 entities in total) |
| Functional Keywords | coiled coil, alpha-helical barrel, nonamer, synthetic, de novo protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 36 |
| Total formula weight | 118447.94 |
| Authors | Shelley, K.L.,Rhys, G.G.,Dawson, W.M.,Brady, R.L.,Woolfson, D.N. (deposition date: 2021-01-12, release date: 2021-04-21, Last modification date: 2024-10-23) |
| Primary citation | Dawson, W.M.,Martin, F.J.O.,Rhys, G.G.,Shelley, K.L.,Brady, R.L.,Woolfson, D.N. Coiled coils 9-to-5: rational de novo design of alpha-helical barrels with tunable oligomeric states. Chem Sci, 12:6923-6928, 2021 Cited by PubMed Abstract: The rational design of linear peptides that assemble controllably and predictably in water is challenging. Short sequences must encode unique target structures and avoid alternative states. However, the non-covalent forces that stabilize and discriminate between states are weak. Nonetheless, for α-helical coiled-coil assemblies considerable progress has been made in rational design. In these, sequence repeats of nominally hydrophobic () and polar () residues, , direct the assembly of amphipathic helices into dimeric to tetrameric bundles. Expanding this pattern to can produce larger α-helical barrels. Here, we show that pentameric to nonameric barrels are accessed by varying the residue at one of the sites. In peptides with four L/I-K-E-I-A-x-Z repeats, decreasing the size of Z from threonine to serine to alanine to glycine gives progressively larger oligomers. X-ray crystal structures of the resulting α-helical barrels rationalize this: side chains at Z point directly into the helical interfaces, and smaller residues allow closer helix contacts and larger assemblies. PubMed: 34745518DOI: 10.1039/d1sc00460c PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.64 Å) |
Structure validation
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