7BHC

Escherichia coli YtfE E125L

Summary for 7BHC

• Entry DOI: 10.2210/pdb7bhc/pdb
• Descriptor: Iron-sulfur cluster repair protein YtfE, FE (III) ION, OXYGEN ATOM, ... (5 entities in total)
• Functional Keywords: di-iron, metal binding protein
• Biological source: Escherichia coli (strain K12)
• Total number of polymer chains: 2
• Total formula weight: 50032.47

Authors

Silva, L.S.O.,Matias, P.M.,Romao, C.V.,Saraiva, L.M. (deposition date: 2021-01-11, release date: 2021-04-21, Last modification date: 2024-01-31)

Primary citation

Silva, L.S.O.,Matias, P.M.,Romao, C.V.,Saraiva, L.M.
Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis.
Front Microbiol, 12:670681-670681, 2021

PubMed Abstract: YtfE is a di-iron protein of the widespread Repair of Iron Centers proteins (RIC) family that has the capacity to donate iron, which is a crucial component of the biogenesis of the ubiquitous family of iron-sulfur proteins. In this work we identify in a previously unrecognized link between the YtfE protein and the major bacterial system for iron-sulfur cluster (ISC) assembly. We show that YtfE establishes protein-protein interactions with the scaffold IscU, where the transient cluster is formed, and the cysteine desulfurase IscS. Moreover, we found that promotion by YtfE of the formation of an Fe-S cluster in IscU requires two glutamates, E125 and E159 in YtfE. Both glutamates form part of the entrance of a protein channel in YtfE that links the di-iron center to the surface. In particular, E125 is crucial for the exit of iron, as a single mutation to leucine closes the channel rendering YtfE inactive for the build-up of Fe-S clusters. Hence, we provide evidence for the key role of RICs as bacterial iron donor proteins involved in the biogenesis of Fe-S clusters.

PubMed: 33995335
DOI: 10.3389/fmicb.2021.670681

Experimental method

X-RAY DIFFRACTION (1.87 Å)