7BFX

deoxyxylose nucleic acid hairpin

Summary for 7BFX

• Entry DOI: 10.2210/pdb7bfx/pdb
• Related: 7BFS
• NMR Information: BMRB: 34588
• Descriptor: dXyNA (5'-D(*(XA)P*(XG)P*(XC)P*(XA)P*(XA)P*(XT)P*(XC)P*(XC)P*(XC)P*(XC)P*(XC)P*(XC)P*(XG)P*(XG)P*(XA)P*(XT)P*(XT)P*(XG)P*(XC)P*T)-3') (1 entity in total)
• Functional Keywords: deoxyxylose nucleic acid, chimeric, dna
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 6054.92

Authors

Mattelaer, C.-A.,Mohitosh, M.,Smets, L.,Maiti, M.,Schepers, G.,Mattelaer, H.-P.,Rosemeyer, H.,Herdewijn, P.,Lescrinier, E. (deposition date: 2021-01-05, release date: 2021-01-27, Last modification date: 2024-01-31)

Primary citation

Mattelaer, C.A.,Maiti, M.,Smets, L.,Maiti, M.,Schepers, G.,Mattelaer, H.P.,Rosemeyer, H.,Herdewijn, P.,Lescrinier, E.
Stable Hairpin Structures Formed by Xylose-Based Nucleic Acids.
Chembiochem, 22:1638-1645, 2021

PubMed Abstract: Xenobiology explores synthetic nucleic acid polymers as alternative carriers of genetic information to expand the central dogma. The xylo- and deoxyxylo-nucleic acids (XyNA and dXyNA), containing 3' epimers of riboses and deoxyriboses, are considered to be potential candidates for an orthogonal system. In this study, thermal and spectroscopic analyses show that XyNA and dXyNA form stable hairpins. The dXyNA hairpin structure determined by NMR spectroscopy contains a flexible loop that locks the stem into a stable ladder-like duplex with marginal right-handed helicity. The reduced flexibility of the dXyNA duplex observed in the stem of the hairpin demonstrates that folding of dXyNA yields more stable structures described so far.

PubMed: 33427360
DOI: 10.1002/cbic.202000803

Experimental method

SOLUTION NMR