7BF0

Zn-bound domain 3 of CDCA1 in complex with carbon dioxide

7BF0 の概要

• エントリーDOI: 10.2210/pdb7bf0/pdb
• 分子名称: Cadmium-specific carbonic anhydrase, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: carbon dioxide, carbonic anhydrase, zinc-bound, marine diatom, lyase
• 由来する生物種: Thalassiosira weissflogii (Marine diatom)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49907.07

構造登録者

Alterio, V.,De Simone, G. (登録日: 2020-12-30, 公開日: 2021-07-14, 最終更新日: 2024-01-31)

主引用文献

Alterio, V.,Langella, E.,Buonanno, M.,Esposito, D.,Nocentini, A.,Berrino, E.,Bua, S.,Polentarutti, M.,Supuran, C.T.,Monti, S.M.,De Simone, G.
Zeta-carbonic anhydrases show CS 2 hydrolase activity: A new metabolic carbon acquisition pathway in diatoms?
Comput Struct Biotechnol J, 19:3427-3436, 2021

PubMed Abstract: CDCA1 is a very peculiar member of the Carbonic Anhydrase (CA) family. It has been the first enzyme to show an efficient utilization of Cd(II) ions in Nature and a unique adaptation capability to live on the surface ocean. Indeed, in this environment, which is extremely depleted in essential metal ions, CDCA1 can utilize Zn(II) or Cd(II) as catalytic metal to support the metabolic needs of fast growing diatoms. In this paper we demonstrate a further catalytic versatility of this enzyme by using a combination of X-ray crystallography, molecular dynamics simulations and enzymatic experiments. First we identified the CO binding site and the way in which this substrate travels from the environment to the enzyme active site. Then, starting from the observation of a structural similarity with the substrate entry route of CS hydrolase from , we hypothesized and demonstrated that also CS is a substrate for CDCA1. This finding is new and unexpected since until now only few CS hydrolases have been characterized, and none of them is reported to have any CO hydratase action. The physiological implications of this supplementary catalytic activity still remain to be unveiled. We suggest here that it could represent another ability of diatoms expressing CDCA1 to adapt to the external environment. Indeed, the ability of this enzyme to convert CS could represent an alternative source of carbon acquisition for diatoms, in addition to CO.

PubMed: 34194668
DOI: 10.1016/j.csbj.2021.05.057

実験手法

X-RAY DIFFRACTION (1.6 Å)