7BBV

Pectate lyase B from Verticillium dahliae

7BBV の概要

• エントリーDOI: 10.2210/pdb7bbv/pdb
• 分子名称: Pectate lyase B, CALCIUM ION, alpha-D-mannopyranose, ... (5 entities in total)
• 機能のキーワード: pectinase, beta-sheets, lyase
• 由来する生物種: Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75903.73

構造登録者

Safran, J.,Habrylo, O.,Bouckaert, J.,Pau Roblot, C.,Senechal, F.,Pelloux, J. (登録日: 2020-12-18, 公開日: 2022-07-13, 最終更新日: 2024-10-23)

主引用文献

Safran, J.,Ung, V.,Bouckaert, J.,Habrylo, O.,Molinie, R.,Fontaine, J.X.,Lemaire, A.,Voxeur, A.,Pilard, S.,Pau-Roblot, C.,Mercadante, D.,Pelloux, J.,Senechal, F.
The specificity of pectate lyase VdPelB from Verticilium dahliae is highlighted by structural, dynamical and biochemical characterizations.
Int.J.Biol.Macromol., 231:123137-123137, 2023

PubMed Abstract: Pectins, complex polysaccharides and major components of the plant primary cell wall, can be degraded by pectate lyases (PLs). PLs cleave glycosidic bonds of homogalacturonans (HG), the main pectic domain, by β-elimination, releasing unsaturated oligogalacturonides (OGs). To understand the catalytic mechanism and structure/function of these enzymes, we characterized VdPelB from Verticillium dahliae. We first solved the crystal structure of VdPelB at 1.2 Å resolution showing that it is a right-handed parallel β-helix structure. Molecular dynamics (MD) simulations further highlighted the dynamics of the enzyme in complex with substrates that vary in their degree of methylesterification, identifying amino acids involved in substrate binding and cleavage of non-methylesterified pectins. We then biochemically characterized wild type and mutated forms of VdPelB. Pectate lyase VdPelB was most active on non-methylesterified pectins, at pH 8.0 in presence of Ca ions. The VdPelB-G125R mutant was most active at pH 9.0 and showed higher relative activity compared to native enzyme. The OGs released by VdPelB differed to that of previously characterized PLs, showing its peculiar specificity in relation to its structure. OGs released from Verticillium-partially tolerant and sensitive flax cultivars differed which could facilitate the identification VdPelB-mediated elicitors of defence responses.

PubMed: 36639075
DOI: 10.1016/j.ijbiomac.2023.123137

実験手法

X-RAY DIFFRACTION (1.2 Å)