7B5J

Anti-CRISPR associated (Aca) protein, Aca2

7B5J の概要

• エントリーDOI: 10.2210/pdb7b5j/pdb
• 分子名称: Anti-CRISPR associated (Aca) protein, Aca2, GLYCEROL (3 entities in total)
• 機能のキーワード: rna-binding, dna-binding, anti-crispr, regulator, rna binding protein, anti-crispr associated (aca) protein, dna binding protein
• 由来する生物種: Pectobacterium phage ZF40
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26511.80

構造登録者

Usher, B.,Birkholz, N.,Fineran, P.C.,Blower, T.R. (登録日: 2020-12-03, 公開日: 2021-06-23, 最終更新日: 2024-06-19)

主引用文献

Usher, B.,Birkholz, N.,Beck, I.N.,Fagerlund, R.D.,Jackson, S.A.,Fineran, P.C.,Blower, T.R.
Crystal structure of the anti-CRISPR repressor Aca2.
J.Struct.Biol., 213:107752-107752, 2021

PubMed Abstract: Bacteria use adaptive CRISPR-Cas immune mechanisms to protect from invasion by bacteriophages and other mobile genetic elements. In response, bacteriophages and mobile genetic elements have co-evolved anti-CRISPR proteins to inhibit the bacterial defense. We and others have previously shown that anti-CRISPR associated (Aca) proteins can regulate this anti-CRISPR counter-attack. Here, we report the first structure of an Aca protein, the Aca2 DNA-binding transcriptional autorepressor from Pectobacterium carotovorum bacteriophage ZF40, determined to 1.34 Å. Aca2 presents a conserved N-terminal helix-turn-helix DNA-binding domain and a previously uncharacterized C-terminal dimerization domain. Dimerization positions the Aca2 recognition helices for insertion into the major grooves of target DNA, supporting its role in regulating anti-CRISPRs. Furthermore, database comparisons identified uncharacterized Aca2 structural homologs in pathogenic bacteria, suggesting that Aca2 represents the first characterized member of a more widespread family of transcriptional regulators.

PubMed: 34116143
DOI: 10.1016/j.jsb.2021.107752

実験手法

X-RAY DIFFRACTION (1.34 Å)