7B21

The X183 domain from Cellvibrio japonicus Cbp2D

これはPDB形式変換不可エントリーです。

7B21 の概要

• エントリーDOI: 10.2210/pdb7b21/pdb
• 分子名称: Carbohydrate binding protein, putative, cbp2D, HEME C, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: x183, c-type cytochrome, cbp2d, cellvibrio japonicus, electron transport
• 由来する生物種: Cellvibrio japonicus (strain Ueda107)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11405.32

構造登録者

Branch, J.,Hemsworth, G.R. (登録日: 2020-11-25, 公開日: 2021-07-21, 最終更新日: 2024-10-16)

主引用文献

Branch, J.,Rajagopal, B.S.,Paradisi, A.,Yates, N.,Lindley, P.J.,Smith, J.,Hollingsworth, K.,Turnbull, W.B.,Henrissat, B.,Parkin, A.,Berry, A.,Hemsworth, G.R.
C-type cytochrome-initiated reduction of bacterial lytic polysaccharide monooxygenases.
Biochem.J., 478:2927-2944, 2021

PubMed Abstract: The release of glucose from lignocellulosic waste for subsequent fermentation into biofuels holds promise for securing humankind's future energy needs. The discovery of a set of copper-dependent enzymes known as lytic polysaccharide monooxygenases (LPMOs) has galvanised new research in this area. LPMOs act by oxidatively introducing chain breaks into cellulose and other polysaccharides, boosting the ability of cellulases to act on the substrate. Although several proteins have been implicated as electron sources in fungal LPMO biochemistry, no equivalent bacterial LPMO electron donors have been previously identified, although the proteins Cbp2D and E from Cellvibrio japonicus have been implicated as potential candidates. Here we analyse a small c-type cytochrome (CjX183) present in Cellvibrio japonicus Cbp2D, and show that it can initiate bacterial CuII/I LPMO reduction and also activate LPMO-catalyzed cellulose-degradation. In the absence of cellulose, CjX183-driven reduction of the LPMO results in less H2O2 production from O2, and correspondingly less oxidative damage to the enzyme than when ascorbate is used as the reducing agent. Significantly, using CjX183 as the activator maintained similar cellulase boosting levels relative to the use of an equivalent amount of ascorbate. Our results therefore add further evidence to the impact that the choice of electron source can have on LPMO action. Furthermore, the study of Cbp2D and other similar proteins may yet reveal new insight into the redox processes governing polysaccharide degradation in bacteria.

PubMed: 34240737
DOI: 10.1042/BCJ20210376

実験手法

X-RAY DIFFRACTION (1.2 Å)